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(Plasmid #113321)


Item Catalog # Description Quantity Price (USD)
Plasmid 113321 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • gRNA/shRNA sequence
  • Species

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer aataggcgtatcacgaggcag
  • 3′ sequencing primer GTTTCGCCACCTCTGACTTG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCD315 was a gift from Jesse Zalatan (Addgene plasmid # 113321 ; ; RRID:Addgene_113321)
  • For your References section:

    Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria. Dong C, Fontana J, Patel A, Carothers JM, Zalatan JG. Nat Commun. 2018 Jun 27;9(1):2489. doi: 10.1038/s41467-018-04901-6. 10.1038/s41467-018-04901-6 PubMed 29950558