|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||113324||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonep15A vector
Vector typeBacterial Expression, CRISPR
Growth in Bacteria
Copy numberLow Copy
Gene/Insert namedCas9 and MCP-SoxS_R93A
- Cloning method Gibson Cloning
- 5′ sequencing primer CACGCATTGATTTGAGTCAGC
- 3′ sequencing primer tcgtaagccatttccgctcg (Common Sequencing Primers)
Plasmid contains a G nucleotide deletion in the BBa_B1002 terminator sequence, which is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJF104B was a gift from Jesse Zalatan (Addgene plasmid # 113324 ; http://n2t.net/addgene:113324 ; RRID:Addgene_113324)
For your References section:Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria. Dong C, Fontana J, Patel A, Carothers JM, Zalatan JG. Nat Commun. 2018 Jun 27;9(1):2489. doi: 10.1038/s41467-018-04901-6. 10.1038/s41467-018-04901-6 PubMed 29950558