pVB3011
(Plasmid #113389)

• Purpose: Galactose-inducible L-BC gag: cure killer
• Depositing Lab: Suzanne Sandmeyer
• Publication: Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528. doi: 10.1371/journal.pgen.1005528. eCollection 2015.

Backbone

• Vector backbone: pYES2.0
• Total vector size (bp): 7954
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: L-BC Gag
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1986
• Mutation: Please see depositor comments

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: Plasmid contains a truncated L-BC gag which is sufficient for curing of L-BC killer