BK alpha subunit
(Plasmid #113566)

• Purpose: mouse BK channel alpha subunit expression vector
• Depositing Lab: Robert Brenner
• Publication: Gruslova et al J Gen Physiol. 2012 Jan;139(1):57-67. doi: 10.1085/jgp.201110698. Epub 2011 Dec 12.

Backbone

• Vector backbone: pCDNA3
• Backbone manufacturer: Invitrogen
• Modifications to backbone: BK alpha includes the MANG (full length) amino terminus
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kcnma1
• Alt name: alpha1
• Species: M. musculus (mouse)
• Insert Size (bp): 4000
• Entrez Gene: Kcnma1 (a.k.a. 5730414M22Rik, BKCA alpha, BKCa, KCa1.1, MaxiK, Slo, Slo1, k(VCA)alpha, mSlo, mSlo1, slo-alpha)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry