pBABE puro mouse IRS-4
(Plasmid #11363)

• Depositing Lab: Ronald Kahn
• Publication: Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Backbone

• Vector backbone: pBABE puro
• Backbone manufacturer: pBABE-puro is available at Addgene
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IRS-4
• Species: M. musculus (mouse)
• Insert Size (bp): 3700
• GenBank ID: NM_010572
• Entrez Gene: Irs4 (a.k.a. IRS-4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SnaBI (destroyed during cloning)
• 3′ cloning site: SnaBI (destroyed during cloning)
• 5′ sequencing primer: pBABE 5

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mouse IRS-4 genomic DNA was screened from the BAC genomic DNA library (Genome Systems Inc. St. Louis, Mo.) by PCR with specific primers for mouse IRS-4 genomic DNA. The AvrII-ClaI fragment encompassing the entire exon 1 of mouse IRS-4 gene was blunt ended and ligated into the SnaBI site in pBABE-puro.