|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11412||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5443
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameRNase HII
Insert Size (bp)690
GenBank IDAAB98116 NP_247101
- Promoter T7
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
Please note that the plasmid pET21a.MJ0135 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET21a.MJ0135 was a gift from Sung-Hou Kim (Addgene plasmid # 11412)
For your References section:Crystal structure of archaeal RNase HII: a homologue of human major RNase H. Lai L, Yokota H, Hung LW, Kim R, Kim SH. Structure. 2000 Aug 15. 8(8):897-904. 10.1016/S0969-2126(00)00179-9 PubMed 10997908