|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11304||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5443
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namesmall heat shock protein
Insert Size (bp)441
GenBank IDAAB98273 Q57733
Entrez GeneMJ_0285 (a.k.a. MJ_0285, MJ0285)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer TAATACGACTCACTATAGGG
- 3′ sequencing primer GCTAGTTATTGCTCAGCGG (Common Sequencing Primers)
Terms and Licenses
Nature. 1998. 394:595-599;
Proc. Natl. Acad. Sciences. 1998. 95:9129-9133
Please note that the plasmid pET21a.MJ0285 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET21a.MJ0285 was a gift from Sung-Hou Kim (Addgene plasmid # 11304)
For your References section:Purification, crystallization, and preliminary X-ray crystallographic data analysis of small heat shock protein homolog from Methanococcus jannaschii, a hyperthermophile. Kim KK, Yokota H, Santoso S, Lerner D, Kim R, Kim SH. J Struct Biol. 1998 Jan . 121(1):76-80. 10.1006/jsbi.1998.3969 PubMed 9573624