|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11413||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5443
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namephosphoserine phosphatase
Insert Size (bp)633
GenBank IDAAB99612 NP_248603 MJ1594
Entrez GeneMJ_1594 (a.k.a. MJ_1594, MJ1594)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer TAATACGACTCACTATAGGG
- 3′ sequencing primer GCTAGTTATTGCTCAGCGG (Common Sequencing Primers)
Terms and Licenses
Please note that the plasmid pET21a.MJ1594 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET21a.MJ1594 was a gift from Sung-Hou Kim (Addgene plasmid # 11413)
For your References section:Crystal structure of phosphoserine phosphatase from Methanococcus jannaschii, a hyperthermophile, at 1.8 A resolution. Wang W, Kim R, Jancarik J, Yokota H, Kim SH. Structure. 2001 Jan 10. 9(1):65-71. 10.1016/S0969-2126(00)00558-X PubMed 11342136