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pET21a.MJ0697
(Plasmid #11411)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11411 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    fibrillarin homolog
  • Alt name
    MJ_0697
  • Species
    M. jannaschii
  • Insert Size (bp)
    690
  • Mutation
    M222T
  • GenBank ID
    AAB98690 NP_247681
  • Entrez Gene
    MJ_0697 (a.k.a. MJ_0697, MJ0697)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Acta Cryst. 1999. D55:338-340.

Please note that the plasmid pET21a.MJ0697 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.

Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a.MJ0697 was a gift from Sung-Hou Kim (Addgene plasmid # 11411)
  • For your References section:

    Crystal structure of a fibrillarin homologue from Methanococcus jannaschii, a hyperthermophile, at 1.6 A resolution. Wang H, Boisvert D, Kim KK, Kim R, Kim SH. EMBO J. 2000 Feb 1. 19(3):317-23. 10.1093/emboj/19.3.317 PubMed 10654930