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pET21a.MJ1247
(Plasmid #11414)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11414 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3-hexulose-6-phosphate isomerase
  • Alt name
    MJ_1247
  • Species
    M. jannaschii
  • Insert Size (bp)
    540
  • GenBank ID
    AAB99251 NP_248242
  • Entrez Gene
    MJ_1247 (a.k.a. MJ_1247, MJ1247)
  • Promoter T7
  • Tag / Fusion Protein
    • T7-tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that the plasmid pET21a.MJ1247 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.

Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a.MJ1247 was a gift from Sung-Hou Kim (Addgene plasmid # 11414)
  • For your References section:

    Crystal structure of MJ1247 protein from M. jannaschii at 2.0 A resolution infers a molecular function of 3-hexulose-6-phosphate isomerase. Martinez-Cruz LA, Dreyer MK, Boisvert DC, Yokota H, Martinez-Chantar ML, Kim R, Kim SH. Structure. 2002 Feb . 10(2):195-204. 10.1016/S0969-2126(02)00701-3 PubMed 11839305