pET21a.MJ1247
(Plasmid #11414)

• Depositing Lab: Sung-Hou Kim
• Publication: Martinez-Cruz et al Structure. 2002 Feb . 10(2):195-204.

Backbone

• Vector backbone: pET21a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5443
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3-hexulose-6-phosphate isomerase
• Alt name: MJ_1247
• Species: M. jannaschii
• Insert Size (bp): 540
• GenBank ID: AAB99251 NP_248242
• Entrez Gene: MJ_1247 (a.k.a. MJ_1247, MJ1247)
• Promoter: T7
• Tag / Fusion Protein:
  • T7-tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the plasmid pET21a.MJ1247 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.

Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244