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pET21a.TM0857
(Plasmid #11419)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 11419 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    flavin binding protein
  • Alt name
    TM0857 riboflavin kinase/FMN adenylyltransferase
  • Species
    T. maritima
  • Insert Size (bp)
    879
  • GenBank ID
    AAD35939 NP_228666 TM0857
  • Entrez Gene
    TM0857 (a.k.a. TM0857)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that the plasmid pET21a.TM0857 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.

Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a.TM0857 was a gift from Sung-Hou Kim (Addgene plasmid # 11419)
  • For your References section:

    Crystal structure of a flavin-binding protein from Thermotoga maritima. Wang W, Kim R, Jancarik J, Yokota H, Kim SH. Proteins. 2003 Sep 1. 52(4):633-5. 10.1002/prot.10353 PubMed 12910462