PurposeExpresses channelrhodopsin-EGFP fusion in CamKIIa+ neurons
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||114216||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 7079
Vector typeMammalian Expression, Mouse Targeting, AAV
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberLow Copy
Gene/Insert nameChannelrhodopsin 2
SpeciesM. musculus (mouse)
Insert Size (bp)1665
- Promoter CamKIIa 1.3 kb
/ Fusion Protein
- EGFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied sequence. The authors have noted that the discrepancies do NOT affect plasmid function or viral packaging.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AAV-CamKIIa-ChR2-EGFP was a gift from Xue Han (Addgene plasmid # 114216 ; http://n2t.net/addgene:114216 ; RRID:Addgene_114216)
For your References section:A MicroRNA-Based Gene-Targeting Tool for Virally Labeling Interneurons in the Rodent Cortex. Keaveney MK, Tseng HA, Ta TL, Gritton HJ, Man HY, Han X. Cell Rep. 2018 Jul 10;24(2):294-303. doi: 10.1016/j.celrep.2018.06.049. 10.1016/j.celrep.2018.06.049 PubMed 29996091