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(Plasmid #114317)


Item Catalog # Description Quantity Price (USD)
Plasmid 114317 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Bmi1 (a.k.a. Bmi-1, Pcgf4)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions. pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCLXEBR-pTF-mBmi1 was a gift from Patrick Salmon (Addgene plasmid # 114317 ; ; RRID:Addgene_114317)
  • For your References section:

    Generation of human cell lines using lentiviral-mediated genetic engineering. Salmon P. Methods Mol Biol. 2013;945:417-48. doi: 10.1007/978-1-62703-125-7_25. 10.1007/978-1-62703-125-7_25 PubMed 23097121