Depositor Sequences: None.
Addgene Sequences: None. Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11433||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5386
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namecell division protein
Insert Size (bp)423
GenBank IDAAB96170 NP_110002 MPN314
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer TAATACGACTCACTATAGGG
- 3′ sequencing primer GCTAGTTATTGCTCAGCGG (Common Sequencing Primers)
Terms and Licenses
Please note that the plasmid pSKB3.MPN314 contains a Kanamycin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSKB3.MPN314 was a gift from Sung-Hou Kim (Addgene plasmid # 11433)
For your References section:Crystal structure of a protein associated with cell division from Mycoplasma pneumoniae (GI: 13508053): a novel fold with a conserved sequence motif. Chen S, Jancrick J, Yokota H, Kim R, Kim SH. Proteins. 2004 Jun 1. 55(4):785-91. 10.1002/prot.10593 PubMed 15146477
Generated by Addgene from plasmid data.