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PLL7.0 tgRFP cPYP
(Plasmid #114464)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 114464 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cPYP
  • Insert Size (bp)
    429
  • Mutation
    M121E
  • Promoter CMV
  • Tag / Fusion Protein
    • tgRFP (N terminal on insert)

Cloning Information

Resource Information

Depositor Comments

Plasmid lacks features necessary for lentiviral production, but should be unaffected for mammalian expression of tgRFP cPYP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PLL7.0 tgRFP cPYP was a gift from Andrew Woolley (Addgene plasmid # 114464 ; http://n2t.net/addgene:114464 ; RRID:Addgene_114464)
  • For your References section:

    Discovering Selective Binders for Photoswitchable Proteins Using Phage Display. Reis JM, Xu X, McDonald S, Woloschuk RM, Jaikaran ASI, Vizeacoumar FS, Woolley GA, Uppalapati M. ACS Synth Biol. 2018 Oct 19;7(10):2355-2364. doi: 10.1021/acssynbio.8b00123. Epub 2018 Sep 27. 10.1021/acssynbio.8b00123 PubMed 30203962