PLL7.0 tgRFP cPYP
PurposeMammalian expression of tgRFP labelled cPYP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||114464||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonePLL 7.0
Vector typeMammalian Expression
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Insert Size (bp)429
- Promoter CMV
/ Fusion Protein
- tgRFP (N terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer GAGCTGTACAAGtcc (Common Sequencing Primers)
Plasmid lacks features necessary for lentiviral production, but should be unaffected for mammalian expression of tgRFP cPYP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PLL7.0 tgRFP cPYP was a gift from Andrew Woolley (Addgene plasmid # 114464 ; http://n2t.net/addgene:114464 ; RRID:Addgene_114464)
For your References section:Discovering Selective Binders for Photoswitchable Proteins Using Phage Display. Reis JM, Xu X, McDonald S, Woloschuk RM, Jaikaran ASI, Vizeacoumar FS, Woolley GA, Uppalapati M. ACS Synth Biol. 2018 Oct 19;7(10):2355-2364. doi: 10.1021/acssynbio.8b00123. Epub 2018 Sep 27. 10.1021/acssynbio.8b00123 PubMed 30203962