Skip to main content
Addgene
Open Menu

A1(R1x2)A3-POX2(100bp)-hrGFP or A1(R1x2)A3
(Plasmid #114573)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114573 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pSL16
  • Backbone size w/o insert (bp) 6055
  • Total vector size (bp) 8031
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    A1(R1x2)A3-POX2-hrGFP
  • Species
    Y. lipolytica, humanized renilla
  • Insert Size (bp)
    1976
  • Promoter A1(R1x2)A3-POX2 hybrid promoter

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer gtaaaacgacggccagt
  • 3′ sequencing primer catgatctcctgcaggc
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    A1(R1x2)A3-POX2(100bp)-hrGFP or A1(R1x2)A3 was a gift from Mark Blenner (Addgene plasmid # 114573)

  • For your References section:

    A Strong Hybrid Fatty Acid Inducible Transcriptional Sensor Built From Yarrowia lipolytica Upstream Activating and Regulatory Sequences. Shabbir Hussain M, Wheeldon I, Blenner MA. Biotechnol J. 2017 Oct;12(10). doi: 10.1002/biot.201700248. Epub 2017 Sep 12. 10.1002/biot.201700248 PubMed 28731568
Related items: