pRS406 ATG9 stopGFP
(Plasmid #114669)

• Purpose: Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag
• Depositing Lab: Daniel Klionsky
• Publication: Feng et al Autophagy. 2016;12(4):648-58. doi: 10.1080/15548627.2016.1157237.

Backbone

• Vector backbone: pRS406
• Backbone size w/o insert (bp): 4297
• Total vector size (bp): 8565
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ATG9
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 4265
• Mutation: Changed the three linker base pairs after the last Arginine to stop codon TAA
• Promoter: ATG9
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (unknown if destroyed)
• 3′ cloning site: Sac1 (unknown if destroyed)
• 5′ sequencing primer: GGT ACT ATT GAC GTC TTG TC
• 3′ sequencing primer: AAG AAA TCC GTA AAG ACG CC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid was cloned by previous lab members.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry