pcDNA3-myc-mE6-AP/2591a
(Plasmid #11468)

• Depositing Lab: Allan Weissman
• Publication: Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-92.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mE6-AP
• Alt name: Ube3a
• Species: M. musculus (mouse)
• Insert Size (bp): 2000
• Mutation: n/a
• GenBank ID: U96636
• Entrez Gene: Ube3a (a.k.a. 4732496B02, 5830462N02Rik, A130086L21Rik, Hpve6a)
• Tag / Fusion Protein:
  • myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn I (not destroyed)
• 3′ cloning site: EcoR V (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH reverse primer

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry