pGEX-KG-UbcH5b/2443
(Plasmid
#11477)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11477 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEX-KG
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Backbone manufacturersee PMID 1852137
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUbcH5b
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Alt nameUbe2d2
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SpeciesH. sapiens (human)
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Insert Size (bp)444
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Mutationn/a
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GenBank IDU39317 NP_003330
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Entrez GeneUBE2D2 (a.k.a. E2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH4, UBCH5B)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ? (unknown if destroyed)
- 3′ cloning site ? (unknown if destroyed)
- 5′ sequencing primer Amersham 5' pGEX
- 3′ sequencing primer Amersham 3' pGEX (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-KG-UbcH5b/2443 was a gift from Allan Weissman (Addgene plasmid # 11477 ; http://n2t.net/addgene:11477 ; RRID:Addgene_11477)