Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #11515)


Item Catalog # Description Quantity Price (USD)
Plasmid 11515 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    huntingtin, exon 1, 46Q glutamines
  • Alt name
    huntingtin (Huntington disease)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    huntingtin exon 1 with 46 glutamines, vector modified-removed N-terminal His tag by digesting vector with MscI and NspV, filling in and blunt-end-ligating back together.
  • GenBank ID
  • Entrez Gene
    HTT (a.k.a. HD, IT15, LOMARS)
  • Tags / Fusion Proteins
    • Trx (N terminal on backbone)
    • His (C terminal on insert)
    • His (out of frame) (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A. Kazantsev
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET32a-HD46Q was a gift from Pamela Bjorkman (Addgene plasmid # 11515 ; ; RRID:Addgene_11515)
  • For your References section:

    Inaugural Article: A linear lattice model for polyglutamine in CAG-expansion diseases. Bennett MJ, Huey-Tubman KE, Herr AB, West AP, Ross SA, Bjorkman PJ. Proc Natl Acad Sci U S A. 2002 Sep 3. 99(18):11634-9. 10.1073/pnas.182393899 PubMed 12193654