pENTR223-PARK7V51G
(Plasmid
#115178)
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PurposeGateway cloning of PARK7V51G into any destination vector
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 115178 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepENTR223
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Backbone manufacturerThermo Scientific
- Backbone size w/o insert (bp) 2790
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Vector typegateway cloning into appropriate destination vectors
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePARK7V51G
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SpeciesH. sapiens (human)
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Insert Size (bp)567
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Mutationp.V51G
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Entrez GenePARK7 (a.k.a. DJ-1, DJ1, GATD2, HEL-S-67p)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer M13_F
- 3′ sequencing primer M13_R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Derived from HsCD00383508 from Harvard PlasmidID. Mutagenesis performed using overlap-extension PCR in combination with Restriction digestion/ligation.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR223-PARK7V51G was a gift from Mohan Babu (Addgene plasmid # 115178 ; http://n2t.net/addgene:115178 ; RRID:Addgene_115178) -
For your References section:
Rewiring of the Human Mitochondrial Interactome during Neuronal Reprogramming Reveals Regulators of the Respirasome and Neurogenesis. Moutaoufik MT, Malty R, Amin S, Zhang Q, Phanse S, Gagarinova A, Zilocchi M, Hoell L, Minic Z, Gagarinova M, Aoki H, Stockwell J, Jessulat M, Goebels F, Broderick K, Scott NE, Vlasblom J, Musso G, Prasad B, Lamantea E, Garavaglia B, Rajput A, Murayama K, Okazaki Y, Foster LJ, Bader GD, Cayabyab FS, Babu M. iScience. 2019 Sep 4;19:1114-1132. doi: 10.1016/j.isci.2019.08.057. 10.1016/j.isci.2019.08.057 PubMed 31536960
