PDF1::mCitrine-MBD
(Plasmid
#115222)
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PurposeMicrotubule visualization
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 115222 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBART27 R4R3
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Vector typePlant Expression
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Selectable markersBasta
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMicrotubule binding domain
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SpeciesM. musculus (mouse)
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Tag
/ Fusion Protein
- mCitrine (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene QC results discovered an IS1 inserted into the plasmid backbone. Please review the sequence for further information.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PDF1::mCitrine-MBD was a gift from Jan Traas (Addgene plasmid # 115222 ; http://n2t.net/addgene:115222 ; RRID:Addgene_115222) -
For your References section:
Transcriptional induction of cell wall remodelling genes is coupled to microtubule-driven growth isotropy at the shoot apex in Arabidopsis. Armezzani A, Abad U, Ali O, Andres Robin A, Vachez L, Larrieu A, Mellerowicz EJ, Taconnat L, Battu V, Stanislas T, Liu M, Vernoux T, Traas J, Sassi M. Development. 2018 Jun 4;145(11). pii: dev.162255. doi: 10.1242/dev.162255. 10.1242/dev.162255 PubMed 29739839
