Depositor Sequences: None.
Addgene Sequences: Partial (2)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11538||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6143
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)498
GenBank IDAAB98941 MJ0936 NP_247931
/ Fusion Protein
- HisTag, non-cleavable (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (destroyed during cloning)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer TAATACGACTCACTATAGGG
- 3′ sequencing primer GTTGCATCACCTTCACCCTCTCC (Common Sequencing Primers)
Terms and Licenses
Please note that the plasmid pLIC.B2.MJ0936 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLIC.B2.MJ0936 was a gift from Sung-Hou Kim (Addgene plasmid # 11538)
For your References section:Structural and functional characterization of a novel phosphodiesterase from Methanococcus jannaschii. Chen S, Yakunin AF, Kuznetsova E, Busso D, Pufan R, Proudfoot M, Kim R, Kim SH. J Biol Chem. 2004 Jul 23. 279(30):31854-62. 10.1074/jbc.M401059200 PubMed 15128743