This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pCMV6-XL4 FLAG-BMP2-Fc
(Plasmid #115771)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 115771 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BMP2
  • Species
    H. sapiens (human)
  • Mutation
    none
  • Entrez Gene
    BMP2 (a.k.a. BDA2, BMP2A, SSFSC)
  • Promoter CMV
  • Tags / Fusion Proteins
    • FLAG (N terminal on backbone)
    • hIgG/Fc (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (not destroyed)
  • 3′ cloning site unknown (not destroyed)
  • 5′ sequencing primer GGA TAG CGG TTT GAC TC
  • 3′ sequencing primer AAA GCC AGA AGA GGG TCT C
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Codon Optimized, FLAG-tag is followed by a NotI site and hIgG-tag is preceeded by a XbaI site. The reverse sequencing primer will sequence the CBLN1 insert only and not the hIgG-tag.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV6-XL4 FLAG-BMP2-Fc was a gift from Davide Comoletti (Addgene plasmid # 115771 ; http://n2t.net/addgene:115771 ; RRID:Addgene_115771)