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HA-DN-14-3-3 zeta (R56A/R60A)
(Plasmid #116889)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 116889 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DN-14-3-3 zeta (R56A/R60A)
  • Species
    H. sapiens (human)
  • Entrez Gene
    YWHAZ (a.k.a. 14-3-3-zeta, HEL-S-3, HEL-S-93, HEL4, KCIP-1, YWHAD)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CCCAAGCTTGATTTAG (SP6)
  • 3′ sequencing primer TTATGCTGAGTGATATC (T7)
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-DN-14-3-3 zeta (R56A/R60A) was a gift from FENG-QIAN Li & Ken-Ichi Takemaru (Addgene plasmid # 116889 ; http://n2t.net/addgene:116889 ; RRID:Addgene_116889)
  • For your References section:

    Chibby cooperates with 14-3-3 to regulate beta-catenin subcellular distribution and signaling activity. Li FQ, Mofunanya A, Harris K, Takemaru K. J Cell Biol. 2008 Jun 30;181(7):1141-54. doi: 10.1083/jcb.200709091. Epub 2008 Jun 23. 10.1083/jcb.200709091 PubMed 18573912