pMO719
(Plasmid
#117479)
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Purpose(Empty Backbone) Desulfovibrio shuttle vector, contains pBG1 and pMB1 replicons, specR
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 117479 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCR8/GW/TOPO
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Backbone manufacturerInvitrogen
- Backbone size (bp) 2817
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Modifications to backbonecaptured pBG1 fragment
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsStable in Desulfovibrio (and other SRB strains). Selection in Desulfovibrio vulgaris Hildenborough is with 100 ug/ml spectinomycin.
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Copy numberHigh Copy
Cloning Information
- Cloning method TOPO Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMO719 was a gift from Judy Wall (Addgene plasmid # 117479 ; http://n2t.net/addgene:117479 ; RRID:Addgene_117479) -
For your References section:
Development of a markerless genetic exchange system for Desulfovibrio vulgaris Hildenborough and its use in generating a strain with increased transformation efficiency. Keller KL, Bender KS, Wall JD. Appl Environ Microbiol. 2009 Dec;75(24):7682-91. doi: 10.1128/AEM.01839-09. Epub 2009 Oct 16. 10.1128/AEM.01839-09 PubMed 19837844
Map uploaded by the depositor.
