pYS2
(Plasmid
#117490)
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PurposeGeneration of unmarked gene deletions in Mycobacterium smegmatis
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 117490 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepYS2
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Vector typeDeletion generation
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namegfp, hyg
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SpeciesM. smegmatis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene QC Next-generation sequencing identified a sequence discrepancy relative to the Genbank file linked under Supplemental Documents. Plasmid is expected to function as described in the associated publication.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYS2 was a gift from Eyal Gur (Addgene plasmid # 117490 ; http://n2t.net/addgene:117490 ; RRID:Addgene_117490) -
For your References section:
Efficient and simple generation of unmarked gene deletions in Mycobacterium smegmatis. Shenkerman Y, Elharar Y, Vishkautzan M, Gur E. Gene. 2014 Jan 1;533(1):374-8. doi: 10.1016/j.gene.2013.09.082. Epub 2013 Oct 4. 10.1016/j.gene.2013.09.082 PubMed 24100088
Map uploaded by the depositor.
