pLex_Cas9
(Plasmid #117987)

• Purpose: pLex_Cas9 is a single insert lentiviral vector expressing Cas9 driven by CMV promoter.
• Depositing Lab: Paul Khavari
• Publication: Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31514-9. doi: 10.1016/j.cell.2018.11.022.

Backbone

• Vector backbone: pLex
• Backbone manufacturer: Thermo Fisher
• Backbone size w/o insert (bp): 9429
• Total vector size (bp): 14070
• Vector type: Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cas9-P2A-NLS-BASTR
• Species: Streptococcus pyogenes
• Insert Size (bp): 4641
• Tag / Fusion Protein:
  • P2A linker, nuclear localization signal and blasticidin resistance gene fused to Cas9 (C terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene was cloned from lentiCRISPRv2 vector (Addgene #52961)
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry