|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||118089||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 7857
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Gene/Insert nameRapamycin-inducible split T7 RNAP with T7 driven mRNA for GFP
SpeciesH. sapiens (human); Evolved genes
- Cloning method Gibson Cloning
- 5′ sequencing primer GTCATGAGATTATCAAAAAGGA
- 3′ sequencing primer TGCATTCATTTTATGTTTCAG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p6-8 was a gift from Bryan Dickinson (Addgene plasmid # 118089 ; http://n2t.net/addgene:118089 ; RRID:Addgene_118089)
For your References section:Evolution of a split RNA polymerase as a versatile biosensor platform. Pu J, Zinkus-Boltz J, Dickinson BC. Nat Chem Biol. 2017 Apr;13(4):432-438. doi: 10.1038/nchembio.2299. Epub 2017 Feb 13. 10.1038/nchembio.2299 PubMed 28192413