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(Plasmid #118085)


Item Catalog # Description Quantity Price (USD)
Plasmid 118085 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer taaacaactaacggacaattctacctaG
  • 3′ sequencing primer CATGCCAGTTCTTTTGGGTATTCC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5-71 was a gift from Bryan Dickinson (Addgene plasmid # 118085 ; ; RRID:Addgene_118085)
  • For your References section:

    Evolution of a split RNA polymerase as a versatile biosensor platform. Pu J, Zinkus-Boltz J, Dickinson BC. Nat Chem Biol. 2017 Apr;13(4):432-438. doi: 10.1038/nchembio.2299. Epub 2017 Feb 13. 10.1038/nchembio.2299 PubMed 28192413