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(Plasmid #118275)


Item Catalog # Description Quantity Price (USD)
Plasmid 118275 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Bong-Kiun Kaang
  • Backbone size w/o insert (bp) 3771
  • Total vector size (bp) 7407
  • Vector type
    AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    XL10 Gold
  • Copy number

Gene/Insert 1

  • Gene/Insert name
  • Alt name
    photoactivated adenylyl cyclase from Beggiatoa sp.
  • Species
    Beggiatoa sp.
  • Insert Size (bp)
  • GenBank ID
  • Promoter Synapsin
  • Tags / Fusion Proteins
    • myc tag (C terminal on insert)
    • His tag (C terminal on insert)
    • mCherry (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Unknown
  • 5′ sequencing primer CGGAGCTACTAACTTCAGCC
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
  • Species
    Spirochaeta thermophila
  • GenBank ID
  • Promoter Synapsin
  • Tag / Fusion Protein
    • P2A (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Unknown
  • 5′ sequencing primer GTTCTCCGTTTGCACTCAGGA
  • 3′ sequencing primer GGGTTCTCCTCCACGTCTC
  • (Common Sequencing Primers)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Syn-FLEX-Sthk-P2A-bPAC_minWPRE was a gift from Dietmar Schmitz (Addgene plasmid # 118275)
  • For your References section:

    Potassium channel-based optogenetic silencing. Bernal Sierra YA, Rost BR, Pofahl M, Fernandes AM, Kopton RA, Moser S, Holtkamp D, Masala N, Beed P, Tukker JJ, Oldani S, Bonigk W, Kohl P, Baier H, Schneider-Warme F, Hegemann P, Beck H, Seifert R, Schmitz D. Nat Commun. 2018 Nov 5;9(1):4611. doi: 10.1038/s41467-018-07038-8. 10.1038/s41467-018-07038-8 PubMed 30397200