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pET23b-Casp9 C287A-His
(Plasmid #11830)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11830 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-23 b
  • Backbone size w/o insert (bp) 3666
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Caspase 9 catalytic mutant
  • Alt name
    Caspase 9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1384
  • Mutation
    C287A
  • Entrez Gene
    CASP9 (a.k.a. APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56)
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Don't Know (unknown if destroyed)
  • 3′ cloning site Don't Know (unknown if destroyed)
  • 5′ sequencing primer T7 terminal primer
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET23b-Casp9 C287A-His was a gift from Guy Salvesen (Addgene plasmid # 11830 ; http://n2t.net/addgene:11830 ; RRID:Addgene_11830)
  • For your References section:

    Caspase-9 can be activated without proteolytic processing. Stennicke HR, Deveraux QL, Humke EW, Reed JC, Dixit VM, Salvesen GS. J Biol Chem. 1999 Mar 26. 274(13):8359-62. 10.1074/jbc.274.13.8359 PubMed 10085063