DENV4 Rluc Reporter GND mutant Replicon
(Plasmid #118586)

• Purpose: For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.
• Depositing Lab: Radhakrishnan Padmanabhan
• Publication: Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8. doi: 10.1099/vir.0.024083-0. Epub 2010 Jul 14.

Backbone

• Vector backbone: pRS424
• Vector type: Yeast-E. col shuttle vector pRS424

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: See PubMed ID: 20631089 for further growth instructions.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
• Species: Replicon sequence is derived from DENV4 cDNA
• Mutation: The conserved GDD in the NS5 polymerase gene mutated to GND to make the replicon RNA derived from it as replication defective

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: described in publication (unknown if destroyed)
• 3′ cloning site: described in publication (unknown if destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry