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DENV4 Rluc Reporter GND mutant Replicon
(Plasmid #118586)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118586 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 118586-D50 50 μg of DNA in Tris buffer $495
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DNA 118586-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pRS424
  • Vector type
    Yeast-E. col shuttle vector pRS424

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    See PubMed ID: 20631089 for further growth instructions.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
  • Species
    Replicon sequence is derived from DENV4 cDNA
  • Mutation
    The conserved GDD in the NS5 polymerase gene mutated to GND to make the replicon RNA derived from it as replication defective

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site described in publication (unknown if destroyed)
  • 3′ cloning site described in publication (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DENV4 Rluc Reporter GND mutant Replicon was a gift from Radhakrishnan Padmanabhan (Addgene plasmid # 118586 ; http://n2t.net/addgene:118586 ; RRID:Addgene_118586)

  • For your References section:

    Construction of a dengue virus type 4 reporter replicon and analysis of temperature-sensitive mutations in non-structural proteins 3 and 5. Alcaraz-Estrada SL, Manzano MI, Del Angel RM, Levis R, Padmanabhan R. J Gen Virol. 2010 Nov;91(Pt 11):2713-8. doi: 10.1099/vir.0.024083-0. Epub 2010 Jul 14. 10.1099/vir.0.024083-0 PubMed 20631089
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