bNS2B47NS3
(Plasmid #155320)

• Purpose: co-expression of DENV4 47 peptides from NS2B cofactor and NS3 full length
• Depositing Lab: Dahai Luo
• Publication: Phoo et al Antiviral Res. 2020 Oct;182:104900. doi: 10.1016/j.antiviral.2020.104900. Epub 2020 Aug 5.

Backbone

• Vector backbone: pNIC
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4779
• Total vector size (bp): 6806
• Modifications to backbone: NA
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Co expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
• Alt name: NS2B47NS3
• Species: Dengue virus 4
• Mutation: WT
• GenBank ID: JF262783 G11337
• Promoter: T7
• Tag / Fusion Protein:
  • His tag (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GTTCTGGCTCGCTGAAACGCCGCTC
• 3′ sequencing primer: gcgcttaatgcgccgctacagggcgcgt

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Ordered from genescript.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry