pBOB-CARMIL2 BH domain-tdTomato
(Plasmid #118737)

• Purpose: To express CARMIL2 tagged BH with tdTomato on C-terminus
• Depositing Lab: John Cooper
• Publication: Lanier et al J Biol Chem. 2016 Jan 15;291(3):1076-91. doi: 10.1074/jbc.M115.676882. Epub 2015 Nov 17.

Backbone

• Vector backbone: pCSC-SP-PW-tdTomato
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CARMIL2 BH domain
• Species: H. sapiens (human)
• Entrez Gene: CARMIL2 (a.k.a. CARMIL2b, IMD58, LRRC16C, RLTPR)
• Promoter: CMV
• Tag / Fusion Protein:
  • tdTomato (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba I (unknown if destroyed)
• 3′ cloning site: Age I (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This tdTomato fusion localizes well to plasma membranes, making it a good marker for fluorescence microscopy experiments.