PurposeTo express membrane targeted tdTomato
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||118739||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameLck domain
SpeciesH. sapiens (human)
MutationH8N (see comments)
Entrez GeneLCK (a.k.a. IMD22, LSK, YT16, p56lck, pp58lck)
- Promoter CMV
/ Fusion Protein
- tdTomato (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Xba I (unknown if destroyed)
- 3′ cloning site Age I (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Cloned membrane targeting Lck sequence from popular Addgene plasmid #61099. H8N mutation does not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBOB-Lck-tdTomato was a gift from John Cooper (Addgene plasmid # 118739 ; http://n2t.net/addgene:118739 ; RRID:Addgene_118739)