Purpose3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the HIV(gag) KK10 epitope "KRWIILGLNK" cloned via BsmBI
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||119053||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerDonald B. Kohn's laboratory at UCLA
- Backbone size w/o insert (bp) 6600
- Total vector size (bp) 9000
Vector typeMammalian Expression, Lentiviral
Selectable markerssurface myc-tag
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Promoter MND
- Cloning method Gibson Cloning
- 5′ sequencing primer GCTCCCCGAGCTCAATAAAAG
- 3′ sequencing primer TGGCTAAGATCTACAGCTGCCTT (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCCLc-MND-B2705-KK10-SABR was a gift from David Baltimore (Addgene plasmid # 119053 ; http://n2t.net/addgene:119053 ; RRID:Addgene_119053)
For your References section:T cell antigen discovery via signaling and antigen-presenting bifunctional receptors. Joglekar AV, Leonard MT, Jeppson JD, Swift M, Li G, Wong S, Peng S, Zaretsky JM, Heath JR, Ribas A, Bethune MT & Baltimore D.. Nature Methods (2019) volume 16, pages 191–198. 10.1038/s41592-018-0304-8