pBS594 promoterless EGFPcre
(Plasmid #11956)

• Depositing Lab: Brian Sauer
• Publication: Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Backbone

• Vector backbone: na
• Backbone size w/o insert (bp): 3900
• Vector type: Mammalian Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP-cre
• Alt name: cre
• Species: bacteriophage P1
• Insert Size (bp): 1800
• Entrez Gene: cre (a.k.a. P1_gp003)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pBS594 differs from pBS592 in having the MT-I A(n) instead of the GCSF A(n). MT-I A(n) is the mouse metallothionein MT-I region including the polyadenylation site and several introns. pBS592 carries the EGFPcre fusion gene in a promoterless vector with several available restriction sites. The GFP moiety of the fusion gene carries the S65T mutation for enhanced fluorescence and is codon-optimised for higher level expression. Two related constructs are the pBS597 with a tet-controlled promoter and the vector pBS598 having constitutive high level expression.