pBS593 promoterless GFPcre
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11959||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4500
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Insert Size (bp)1850
Entrez Genecre (a.k.a. P1_gp003)
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pBS593 differs from pBS532 in having the MT-I A(n) instead of the GCSF A(n). MT-I A(n) is the mouse metallothionein MT-I region including the polyadenylation site and several introns. pBS532 carries a GFPcre fusion gene. The GFP moiety carries the S65T mutation for enhanced fluorescence, but is not codon-optimized for mammalian expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS593 promoterless GFPcre was a gift from Brian Sauer (Addgene plasmid # 11959 ; http://n2t.net/addgene:11959 ; RRID:Addgene_11959)
For your References section:GFPcre fusion vectors with enhanced expression. Le Y, Miller JL, Sauer B. Anal Biochem. 1999 Jun 1. 270(2):334-6. 10.1006/abio.1999.4110 PubMed 10334853