pAM5057
(Plasmid
#120085)
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PurposeBroad host range plasmid. Theophylline inducible expression of YFP in various cyanobacteria.
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Depositing Lab
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Depositing OrganizationUniversity of California, San Diego (UCSD)
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 120085 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 120085-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 120085-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneRSF1010Y25F-AADA
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Vector typeOther
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin and Streptomycin, 50 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter
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SpeciesBroad range of genetically tractable cyanobacteria
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 120085-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 120085-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAM5057 was a gift from Susan Golden (Addgene plasmid # 120085 ; http://n2t.net/addgene:120085 ; RRID:Addgene_120085) -
For your References section:
Regulation of gene expression in diverse cyanobacterial species by using theophylline-responsive riboswitches. Ma AT, Schmidt CM, Golden JW. Appl Environ Microbiol. 2014 Nov;80(21):6704-13. doi: 10.1128/AEM.01697-14. Epub 2014 Aug 22. 10.1128/AEM.01697-14 PubMed 25149516
