pRL488
(Plasmid
#70263)
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Purpose(Empty Backbone) designed to facilitate transcriptional fusions to luxAB, encoding luciferase, on a plasmid that can replicate in both Escherichia coli and strains of filamentous cyanobacteria.
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Depositing Lab
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Depositing OrganizationMichigan State University
Located in United States of America -
Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 70263 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepRL25
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Backbone manufacturerWolk Lab, Addgene Plasmid #70253
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Modifications to backboneadded luxAB
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Vector typeLuciferase ; cyanobacteria expression
- Promoter none
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- luxAB (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
MCS: SbfI-SalI-XmaI-KpnI-SacI
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRL488 was a gift from Peter Wolk (Addgene plasmid # 70263 ; http://n2t.net/addgene:70263 ; RRID:Addgene_70263) -
For your References section:
Developmental regulation and spatial pattern of expression of the structural genes for nitrogenase in the cyanobacterium Anabaena. Elhai J, Wolk CP. EMBO J. 1990 Oct;9(10):3379-88. PubMed 2120040
Map uploaded by the depositor.
