pRL1063a
(Plasmid #70687)

• Purpose: pRL1058 with Vibrio fischeri luxAB for sensing
• Depositing Lab: Peter Wolk
• Publication: Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5355-9.

Backbone

• Vector backbone: pRL1058
• Backbone manufacturer: Wolk Lab
• Modifications to backbone: 7835 bp of the 8939 bp are a transposon whose ends are linked by an origin of transfer
• Selectable markers: Bleomycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, Streptomycin, Bleomycin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Transposon Tn5-1063
• GenBank ID: pRL1063a as a whole is presented in GenBank: U55385.2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Tn5 derivative Tn5-1063a (in plasmid pRL1063a) contains a p15A oriV, and its antibiotic-resistance determinants are driven by an Amaranthus hybridus ribulose bisphosphate carboxylase promoter (see pRL439 and pRL1058 is this series of plasmids), providing large numbers of transposon mutants. Plasmid pJE205 bearing the luxA and luxB genes of Vibrio fischeri was the kind gift of M. Silverman, Agouron Institute, La Jolla, CA. The sequence of luxA gene in this construct has been updated and differs slightly from the original X06758.1 sequence.

Additional references:
Huang G, Fan Q, Lechno-Yossef S, Wojciuch E, Wolk CP, Kaneko T, Tabata S (2005) Clustered genes required for the synthesis of heterocyst envelope polysaccharide in Anabaena sp. strain PCC 7120. J Bacteriol 187: 1114-1123
Fan Q, Huang G, Lechno-Yossef S, Wolk CP, Kaneko T, Tabata S (2005) Clustered genes required for synthesis and deposition of envelope glycolipids in Anabaena sp. strain PCC 7120. Mol Microbiol 58: 227-243