PurposepCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||120225||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 6765
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberLow Copy
SpeciesSynthetic; E. coli
Insert Size (bp)4200
- Promoter pTet
/ Fusion Protein
- ssrA (C terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer gttgacactctatcgttgat (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCas9CR4-NG was a gift from Christopher Reisch (Addgene plasmid # 120225 ; http://n2t.net/addgene:120225 ; RRID:Addgene_120225)