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Christopher Reisch Lab: pCas9CR4 variants for increased targeting space
Unpublished

Plasmids from Article

ID Plasmid Purpose  
120224pCas9CR4-VQRpCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015
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120225pCas9CR4-NGpCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.
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120227pCas9CR5pCas9CR4 with stop codon to prevent translation of ssrA degradation tag
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120228pCas9CR5-NGpCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.
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