PurposepCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||120224||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 6770
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberLow Copy
SpeciesSynthetic; E. coli
Insert Size (bp)4200
- Promoter pTet
/ Fusion Protein
- ssrA (C terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer gttgacactctatcgttgat (Common Sequencing Primers)
The TetR protein coding sequence contains an F178S difference compared to the reference sequence that does not impact the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCas9CR4-VQR was a gift from Christopher Reisch (Addgene plasmid # 120224 ; http://n2t.net/addgene:120224 ; RRID:Addgene_120224)