pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE
(Plasmid
#120268)
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PurposePermits retrograde transsynaptic spread by driving Cre-dependent co-expression of the RbG plus a nuclear reporter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 120268 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
This item is currently unavailable outside the US without additional regulatory approval.
A non-refundable shipping export licensing fee of $85 is required to cover Addgene’s additional processing costs.
Backbone
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Vector backboneUnknown
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE was a gift from Edward Callaway (Addgene plasmid # 120268 ; http://n2t.net/addgene:120268 ; RRID:Addgene_120268) -
For your References section:
Afferent Inputs to Neurotransmitter-Defined Cell Types in the Ventral Tegmental Area. Faget L, Osakada F, Duan J, Ressler R, Johnson AB, Proudfoot JA, Yoo JH, Callaway EM, Hnasko TS. Cell Rep. 2016 Jun 21;15(12):2796-808. doi: 10.1016/j.celrep.2016.05.057. Epub 2016 Jun 9. 10.1016/j.celrep.2016.05.057 PubMed 27292633
