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(Plasmid #120720)


Item Catalog # Description Quantity Price (USD)
Plasmid 120720 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 7649
  • Total vector size (bp) 7700
  • Vector type
    Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
    Esr1 shRNA
  • Alt name
    Estrogen receptor alpha, ERa
  • gRNA/shRNA sequence
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Esr1 (a.k.a. ER, ER-alpha, ERa, ERalpha, ESR, Estr, Estra, Nr3a1)
  • Promoter Mouse U6
  • Tag / Fusion Protein
    • GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer U6
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The 19 nucleotide targeting sequence for Esr1 was obtained from this publication:

This plasmid is also featured in the following article:
(J Neurosci. 2020 )

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-shEsr1 was a gift from Amy Lasek (Addgene plasmid # 120720 ; ; RRID:Addgene_120720)
  • For your References section:

    Estrogen Receptor beta in the Nucleus Accumbens Regulates the Rewarding Properties of Cocaine in Female Mice. Satta R, Certa B, He D, Lasek AW. Int J Neuropsychopharmacol. 2018 Apr 1;21(4):382-392. doi: 10.1093/ijnp/pyx118. 10.1093/ijnp/pyx118 PubMed 29294029