PurposeMoClo golden gate assembly AB part for pLbda-CasRQi (strong lambda promoter with downstream dCas binding site for Qi-lab gRNA (UC16m); gRNA sequence taken from DOI: 10.1016/j.cell.2013.02.022).
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||120946||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2100
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)99
- Cloning method Gibson Cloning
- 5′ sequencing primer AAATAGGCGTATCACGAGGCA
- 3′ sequencing primer TTACCGCCTTTGAGTGAGCTG (Common Sequencing Primers)
Very strong promoter! To avoid mutations, grow this plasmid in KL740 E. coli, or another strain containing lambda cI. If in KL740, grow at 30degreesC. Please see Supplemental Documents for annotated Genbank file.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:P35m was a gift from Richard Murray (Addgene plasmid # 120946 ; http://n2t.net/addgene:120946 ; RRID:Addgene_120946)