p15aC-3D1D-5
(Plasmid #121050)

• Purpose: Bacterial expression of PIS, PI4K and PI3P5K mutant E545K
• Depositing Lab: Sanford Simon
• Publication: Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi: 10.1126/sciadv.aat4872. eCollection 2019 Mar.

Backbone

• Vector backbone: p15aC
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: PIS
• Alt name: phosphatidyl inositol synthase
• Alt name: phosphatidylinositol synthase
• Alt name: PI synthase
• Species: Trypanosome brucei
• Tag / Fusion Protein:
  • myc (N terminal on insert)

Gene/Insert 2

• Gene/Insert name: phosphatidylinositol 4-phosphate 5-kinase
• Alt name: phosphatidylinositol 4-phosphate 5-kinase type-1 α isoform 2
• Alt name: PI4P5Kα
• Alt name: PI4P5K
• Species: H. sapiens (human)
• Tag / Fusion Protein:
  • myc (N terminal on insert)

Gene/Insert 3

• Gene/Insert name: phosphatidylinositol 3-kinase catalytic subunit p110α of class IA E545K
• Alt name: PI3K-E545K
• Alt name: phosphatidyl inositol 4-kinase β
• Alt name: PI4Kβ
• Species: Homo sapiens
• Tag / Fusion Protein:
  • none

Resource Information

• Supplemental Documents:
  • p15aC-3D1D-5.gbk
  • annotated GenBank file
• A portion of this plasmid was derived from a plasmid made by: RCAS(A) human p110 alpha E545K (Plasmid #52211)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry