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Escherichia coli as a platform for the study of phosphoinositide biology.
Botero S, Chiaroni-Clarke R, Simon SM
Sci Adv. 2019 Mar 27;5(3):eaat4872. doi: 10.1126/sciadv.aat4872. eCollection 2019 Mar.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
107862p15aC-GFPControl plasmid expressing GFP
107863p15aC-1DBacterial expression of PIS
107864p15aC-4D1DBacterial expression of PIS and PI4K
107865p15aC-1D-5Bacterial expression of PIS and PI4P5K
107866p15aC-4D1D-5Bacterial expression of PIS, PI4K and PI4P5K
107867pClodAcytCh-PIP2PHGFPBacterial expression of Cherry and GFP-PLCδ-PH domain
107870pClodANL-CherryBacterial expression of cherry-NanoLuc
107871pClodANL-FGF2Bacterial expression of FGF2-NanoLuc
107872pClodANL-FGF2YEBacterial expression of FGF2phosphomimic-NanoLuc
107873pClodANL-FGF2NBAABacterial expression of FGF2 minimum export mutant-NanoLuc
107874pClodANL-FGF4Bacterial expression of FGF4-NanoLuc
113346pClodAcytCh-GFP-P4MBacterial expression of Cherry and GFP-SidM-P4M domain (2 copies)
121050p15aC-3D1D-5Bacterial expression of PIS, PI4K and PI3P5K mutant E545K

Antibodies from Article